University of Delaware 2 credits MMSC
This laboratory course introduces students to the clinical/diagnostic applications of molecular techniques. The course will provide hands-on experience in using biochemical and imaging-based assays for molecular diagnostics. Laboratory sessions include discussion, demonstration and hands-on practice of: isolation of nucleic acids from biological samples, use of hybridization-based assays in diagnostic procedures, preparation of probes, the clinical application of PCR, RT-PCR and Flow cytometry in diagnosis/prognoses of diseases. Students will be introduced to DNA and RNA sequencing platforms and liquid biopsy for analysis of genetic polymorphisms and genetic screening will be provided. Prerequisite: BISC 205 or BISC 207 and BISC 217 ; and BISC 208 and BISC 218 .
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