Stony Brook University 3 credits HDO
This course aims to introduce theoretical principles and experimental techniques used for laboratory investigation in biomedical research. Students will familiarize themselves with the instrumentation and techniques used to investigate different molecular and cell biological problems through a combination of lectures and demonstrations. Various topics will be covered such as tissue culture and isolation and characterization of DNA, RNA and proteins using various techniques. Students will be introduced to recombinant DNA technology including cloning in various non-viral and viral vectors that allow modulation of gene expression. Transgenic mice technology and Cre-lox system and gene editing technologies will be introduced. In addition, the use of mass spectrometry, microarray and DNA deep sequencing to understand biological processes will be discussed.. 3 credits Grading Letter graded (A, A-, B+, etc.)
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