Temple University 3 credits BIOL
Genome editing as such is not that new. Scientists have been "editing" genomes of bacteria, yeast and the mouse for some 30 years. Several recent technical advances, however, have pushed genome editing to the forefront of biological research. The first is discovery and development of nucleases, CRISPR/Cas9 in particular, which can be directed to cut DNA at just about any location. The second factor is a combination of stem cell technologies. They make it possible to produce stem cell out of many different tissues, which can then in turn be differentiated into various cell types, or carry out editing in the zygote. This will be a fast-paced seminar-like class. We will read and discuss primary research papers which describe advances relevant to the genome editing field, culminating in their application to edit the genomes of large mammals including the species Homo sapiens.
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