New York University 3 credits Bioinformatics
Screening of differential expression of genes using microarray technology builds the opportunities for personalized medicine converging soon to medical informatics and to our health care system. The course will start with a discussion of gene expression biology, presenting microarray platforms, design of experiments, and Affymetrix file structures and data storage. R programming is introduced for the preprocessing Affymetrix data for Image analysis, quality control and array normalization, log transformation and putting the data together. Bioconductor software will be dealt with data importing, filtering, annotation and analysis. Machine learning concepts and tools for statistical genomics will be addressed along with distance concept, cluster analysis, heat map and class discovery. Case studies link the methodology to biomolecular pathways, gene ontology, genome browsing and drug signatures.
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